Ross and Turkewitz (1982) and Ross-Kossak and Turkewitz (1986) proposed a hypothesis of sequential changes in hemispheric advantage with increase of familiarity for unfamiliar stimulus recognition. This hypothesis was examined by three experiments. Subjects were given a task involving recognition of unfamiliar visual materials, human faces and unknown letter, which were tachistoscopically presented to the left or the right visual field. The hypothesis was not confirmed with both face and unknown letter stimuli. Based on these findings, effects of familiarization of stimulus materials were discussed.